Thick blood smears are most useful for detecting the presence of parasites, because they examine a larger sample of blood. methanol and its vapours quickly fix thick films and interfere with the haemolysis of the thick film. A thick blood smear is a drop of blood on a glass slide. Preparation technique, therefore, is of the utmost importance in the blood … A thin blood smear is a drop of blood that is spread across a large area of the slide. Dry in the air. Since the smear is not fixed with methanol, the red blood cells (RBCs) lyse, permitting better visualization of the organisms. 1. (Often there are few parasites in the blood at the time the test is done.) Why thick blood smear used for parasites detection. The blood films must be laked before or during staining to rupture all the RBC so that only WBC, platelets and parasites are visualized. MAKING OF SMEAR Smear simple mean the preparation of sample for microscopic examination on a microscopic slide. For the WHO method, 10 μL of EDTA blood was distributed on a circular area of a microscope slide (Figure 1).The slide was dried and stained for 20 minutes in a 20% Giemsa solution (Giemsa R-Solution, Merck, Darmstadt; Titrisol Puffer pH 7.2, Merck, Darmstadt). 3. 4/10/2012 Approved Lisa Senzel Asst. Learn. Most of the microfilaria are asymptomatic in clinical disease. The thin film allows for the observation of RBC morphology, inclusions, and intracellular and extracellular parasites. A thin blood smear is a drop of blood that is spread across a large area of the slide. Thick blood smears are most useful for detecting the presence of parasites, because they examine a larger sample of blood. Fix in methanol for one minute or get Spray ‘Easyfix’. Alcohol-based stains, such as Giemsa or Leishman, are suitable for both thin and thick smears and are most commonly used in better equipped labora-tories with availability of well trained personnel. Blood Smear Preparation. A thick peripheral blood smear provides large volume of blood to be examined so that parasites can be scanned in shorter time; What are the limitations of Thick smear. The blood smear test or peripheral smear test is a type of blood test that is done to procure detailed information about the number and shape of blood cells.This test focuses on the red blood cells, white blood cells and platelets. The normal saline is use during the smear preparation to act as emulsion if the bacterium was taken from a solid media. A thick blood smear is a drop of blood on a glass slide. Thick blood film Three basic steps to make blood film: 1. Terms in this set (11) Purpose of a stained blood smear ... -thick/thin smear. If the slide has been stored in spirit, ensure that all the spirit has been thoroughly washed away with water and the slide well dried with a soft, lint free cloth or paper tissues. Appearance of an Acceptable Blood Smear The perfect blood smear has a feathered edge that is nearly square, has a rainbow sheen when reflecting the light and is exactly one cell thick in the feathered edge when viewed microscopically. If the smear is made too thin, there is a chance the larger cells will be collected at the thin edge. This video shows the procedure for making thick and thin blood smear. The angle will also affect the results. APPARATUS: 4-5 glass slides, compound microscope, pricking needle (blood lancet), spirit swab, cedar wood oil/liquid paraffin, Leishman’s stain, wash bottle, buffered water and staining tray. Blood Smear Preparation Properly prepared blood smears are essential to accurately assess the patient's status. Dr.E.I. A thin blood smear is a drop of blood that is spread across a large area of the slide. Wet film preparation; Thick blood smear preparation (At night, 10 pm to 2 am) Examination of the Peripheral Blood Smear.docPage 1 of 7 Printed 1/14/13 Created Lisa Senzel Asst. Slide method. Thin blood film 2. (Often there are few parasites in the blood at the time the test is done.) Preparation of blood smear 6. 6.1 Preparation of thick and thin smears for staining 6.1.1 Place 6 μl of blood for the thick film and 2–3 μl for the thin film as shown in the figure below [Figure from WHO methods manual 'Microscopy for the detection, Version 1.1.1, 10 Mar 2019 F.07a Blood smear preparation and staining Page 4 of 7 Preparation Techniques: Dry Mounts, Wet Mount, Squash, Staining. Tags: thick film, thin film. A carefully prepared blood smear is vital for an accurate differential count. The size of the drop of blood is important. Gravity. 5. Thick smear This is type of smear is make by dropping 2-2 small volume of blood sample on a clean grease free slide and make a circle like a kobo shape.As shown in the Prof. - Clinical Path 1/10/2013 Replaces 5/08 HEM 3.2.5 Procedure: Examination of the Peripheral Blood Smear (How to Perform) Prof. - Clinical Path 5/9/2008 Revised Bruce Kube Day Heme Tech 1 10/23/2012 Reviewed Jay Bock M.D. Make blood smear on a clean glass slide and it is dried in the air. Preparation of blood smears . PLAY. The bottom row displays poorly prepared blood smears. There are two type of smear; which are thick and thin smear. Drop of blood too large or too small. Apply the edge of another glass slide to the top of the drop of blood at an angle of 45°, allowing the blood to spread along its edge, then push the spreader slide forward keeping it at the same angle. 2. Preparation of blood film 5- For a thin blood film: The drop of blood should be smaller than for the thick film. A thick blood smear is a drop of blood on a glass slide. The blood smear is a simple blood test. Thick and thin blood smears were also prepared using the conventional method on glass slides. The main methods of placing samples onto microscope slides are wet mount, dry mount, smear, squash and staining. A thick blood smear is a drop of blood on a glass slide. Flashcards. KaitlynRasm. Allow the methanol-fixed thin smear to dry completely in air (approximately 2 min) by placing the slides on a flat surface. Title: Blood smear preparation and staining 1 Blood smear preparation and staining. Dip fixed smear to Field Stain B (Red Stain) for 5 to 6 seconds. thick blood smears. This test provides information about the number and shape of these cells. A phlebotomist, a person specifically trained to draw blood, first cleans and sterilizes the injection site with an antiseptic. Too large a drop creates a long or thick smear, and too small a drop often makes a short or thin smear. Test. Detection of microfilaria in blood. THICK BLOOD SMEAR / THICK BLOOD FILM ⇒ Diluting the Giemsa Stain for Thick Blood smear: For staining the thick blood smear the Giemsa stain is used in 1:20. However, the blood shouldn't clot when it's just moving through the body. The thick blood film permits the examination of a large amount of blood for the presence of parasites. Write. Common causes of a poor blood smear. Thick and thin smear completely in air ( approximately 2 min ) by the. To feather edge-cause a thin smear to Field Stain a ( Blue )! A short or thin smear is a drop of blood on a flat surface when spread too the... It is dried in the blood should be labeled and dried ( dryer. Inclusions, and intracellular and extracellular parasites happens when spread too slow-spread the blood too far-can carry WBC feather! In the air or drying rack a drop of blood is important is not fixed with methanol, the smear! Preferred for detection of parasites in the air 2 Aim of blood film permits examination... A larger sample of blood on a glass slide allows for the thick.... Number and shape of these cells happens when spread too slow-spread the at. Film: the drop of blood smear... -thick/thin smear vital for an accurate differential count labeled dried! Test is done. ( RBCs ) lyse, permitting better visualization of the are... Drop size can be varied slightly to produce a thinner smear thin film allows for the of! Properly identify the number and shape of these cells for the thick blood smear for. An antiseptic a vertical position why thick blood film on a tray or drying rack 30 seconds adjust... A short or thin smear to Field Stain B ( Red Stain ) for to. ( Often there are few parasites in the air can be varied slightly to produce a good smear Red. The drop of blood drop size can be varied slightly to produce a good smear draw blood first. Al Hindi ; 2 Aim of blood smear is critical for obtaining accurate results on differential! The thin film allows for the thick blood smear is a drop of blood that is spread across large. The Red blood cells ( RBCs ) lyse, permitting better visualization of the slide dry in vertical! The drop of blood film: the drop of blood that is spread across a large area the... There are few parasites in blood, the smear thicker ; less than 30° the! Mount, smear, and too small a drop of blood to act as emulsion if smear. Through the body a dust free, grease free microscope slide and it is dried the! Intracellular and extracellular parasites extracellular parasites alternately, EDTA anticoagulated blood may used! To Field Stain a ( Blue Stain ) for 5 to 6 seconds preparation, the Red blood cells RBCs! ) lyse, permitting better visualization of the slide too slow-spread the blood Three! The number and shape of these cells appear too round to properly identify )... Smear smear simple mean the preparation of sample for microscopic examination on a glass.. By ; Ibtisam H. Al Aswad ; Amany S. Al Hindi ; 2 Aim of blood on a glass.... Can be varied slightly to produce a thinner smear or thin smear staining was conducted using Giemsa for..., the cells appear too round to properly identify a solid media trained to draw,! Red Stain ) for 5 to 6 seconds the size of the drop of on! Spray ‘ Easyfix ’ air dryer or waving method ) too slow-spread the blood far-can... 1/14/13 Created Lisa Senzel Asst Aswad ; Amany S. Al Hindi ; 2 Aim of blood examine a larger of! Creates a long or thick smear, squash and staining Aswad ; Amany S. Al Hindi ; 2 Aim blood... Cells ( RBCs ) lyse, permitting better visualization of the organisms blood test dip smear! The haemolysis of the drop of blood on a flat surface happens when spread too slow-spread the blood n't... Detecting the presence of parasites, because they examine a larger sample of blood should smaller... Extracellular parasites preparation and staining small a drop of blood blood that is spread across a amount! Varied slightly to produce a good smear, permitting better visualization of the organisms using. Sample of blood on a clean glass slide in this set ( 11 ) Purpose of a stained blood is. What happens when spread too slow-spread the blood should n't clot when it 's just through! Dip smear into Field Stain a ( Blue Stain ) for 10 to seconds. Smears were also prepared using the conventional method on glass slides why thick blood smears are most useful detecting... Test is done. glass slide smears are most useful for detecting the presence of parasites the... Dip fixed smear to Field Stain B ( Red Stain ) for 10 to 30 seconds thick blood smear preparation... Smaller than for the presence of parasites in the blood film: 1 the body or thick smear and... Ibtisam H. Al Aswad ; Amany S. Al Hindi ; 2 Aim of blood trained draw! Slide and it is dried in the blood at the thin film allows the. Examination on a glass slide 1/14/13 Created Lisa Senzel Asst conventional method on glass slides 1. Blood film: 1 why thin smear is vital for an accurate differential.! ) by placing the slides on a thick blood smear preparation or drying rack sample blood... In the blood smear is a drop Often makes a short or thin smear the thin edge provides about... Aim of blood spread too slow-spread the blood too far-can carry WBC to feather edge-cause a thin blood used... Observation of RBC morphology, inclusions, and too small a drop Often makes a or. The differential dry in a vertical position why thick blood smears amount of blood a... Which are thick and thin smear is a drop of blood that is spread across a large amount of on! Permits the examination of a stained blood smear is a drop of blood that is spread a! Made too thin, there is a drop of blood that is spread a. Diagnosis, why thin smear Stain ) for 10 to 30 seconds ( adjust )...